期刊论文

Simultaneous determination of three constituents in Qingjie Granules by QAMS

中文

中成药

1001-1528

2019

41

9

2052-2057

10.3969/j.issn.1001-1528.2019.09.007

湖南中医药大学中医学国内一流建设学科(2018) 全国名老中医药专家传承工作室建设项目(国中医药人教发[2016]42).

本校为第一机构

目的建立一测多评法同时测定清解颗粒(苦杏仁、防风、石膏等)中3种成分的含有量。方法该药物甲醇提取液的分析采用依利特C_(18)色谱柱(4.6 mm×250 mm,5 μm);流动相甲醇-乙腈-0.1%冰醋酸,梯度洗脱;体积流量1 mL/min;检测波长210、254 nm;柱温30 ℃。以苦杏仁苷为内标,建立其他2种成分的相对校正因子,测定含有量。结果苦杏仁苷、升麻素苷、5-O-甲基维斯阿米醇苷分别在37.08~ 1 236、22.4 ~ 748、11.88 ~ 396 μg/mL范围内线性关系良好(R~2≥0.999 9),平均加样回收率102.17%、101.12%、104.73%,RSD 1.85%、1.74%、2.12%。一测多评法所得结果与外标法接近。结论该方法准确可靠,可用于清解颗粒的质...

AIM To establish a quantitative analysis of multi-components by single-marker(QAMS) method for the simultaneous content determination of three constituents in Qingjie Granules(Armeniacae Semen Amarum, Saposhnikoviae Radix, Gypsum Fibrosum, etc.).METHODS The analysis of methanol extract of this drug was performed on a 30 ℃ thermostatic EliteC18 column(4.6 mm×250 mm, 5 μm), with the mobile phase comprising of methanol-acetonitrile-0.1% glacial acetic acid flowing at 1 mL/minin a gradient elution manner, and the detection wavelengths were set a...