期刊论文

Effects of Danggui Shaoyao Powder medicated serum on the cycle and apoptosis of Aβ_(1-42)-induced SH-SY5Y cells

中文

中成药

1001-1528

2020

42

11

2875-2882

10.3969/j.issn.1001-1528.2020.11.010

81774129:国家自然科学基金 2018JJ2296:湖南省自然科学基金面上项目 2019JJ50441:湖南省自然科学基金面上项目 2018025:湖南省中医药局科研项目 19K065:湖南省教育厅科学研究项目 18B246:湖南省教育厅科学研究项目

本校为第一机构

目的研究当归芍药散含药血清对Aβ_(1-42)诱导的人神经母细胞瘤细胞( SH-SY5Y)细胞周期和细胞凋亡的调控作用。方法利用Aβ_(1-42)( 10 μmol /L)处理SH-SY5Y细胞24 h构建AD细胞模型,给予不同浓度当归芍药散含药血清( 2.5%、5%、10%)干预24 h。分别采用MTT、流式细胞术和免疫荧光分别检测细胞存活率、细胞周期和细胞凋亡;采用Western blot检测凋亡相关蛋白Bax、Bcl-2和细胞周期蛋白D1( Cyclin D1)表达。结果与空白对照组比较,Aβ_(1-42)处理后SH-SY5Y细胞存活率下降,凋亡率增高,细胞周期S期细胞数增加,G_2 /M期细胞数量减少( P< 0.01);与模型组比较,给予5%、10%当归芍药散含药血清干预后,细胞存...

AIM To investigate the regulatory effect of Danggui Shaoyao Powder medicated serum on the cycle and apoptosis of Aβ_(1-42)-induced SH-SY5Y cells. METHODS SH-SY5Y cells exposed to 24 hours Aβ_(1-42) ( 10 μmol /L) were developed into Alzheimer’s disease cell models for another 24 hours treatment of different concentrations of Danggui Shaoyao Powder medicated serum ( 2.5%,5%,10%). The cells were subjected to detection of cell viability,cell cycle and apoptosis by MTT,flow cytometry and immunofluorescence; and determination of the expression of...