期刊论文

Protective effects of paeoniflorin on CoCl_2-induced oxidative stress injury of PC12 cells

中文

中成药

1001-1528

2020

42

10

2605-2610

10.3969/j.issn.1001-1528.2020.10.012

湖南中医药大学中医学国内一流建设学科资助项目(2018) 湖南中医药大学中医学一级学科开放性基金项目(2018ZYX48)。

本校为第一机构

目的研究芍药苷对氯化钴诱导PC12细胞氧化应激损伤的保护作用。方法芍药苷预先处理PC12细胞1 h,再加入CoCl_2共孵育24 h。采用MTT法检测PC12细胞存活率,利用DCFH-DA探针、Annexin FITC-V/PI双染法、JC- 1探针检测芍药苷(100、200 μg /mL)对PC12细胞ROS水平、细胞凋亡率及线粒体膜电位值(MMP)的影响。结果CoCl_2诱导PC12细胞24 h后,其半数抑制浓度(IC_(50) )为1.2 mmol /L。与模型组比较,200 μg /mL芍药苷提高CoCl_2诱导PC12细胞存活率(P

AIM To study the protective effects of paeoniflorin on CoCl_2-induced oxidative stress injury of PC12 cells. METHODS PC12 cells previously subjected to 1h paeoniflorin pre-treatment were incubated with CoCl_2 for 24 h in vitro. PC12 cells had their survival rate detected by MTT method; and their ROS level,cell apoptosis rate and mitochondrial membrane potential (MMP) due to the effect of paeoniflorin (100,200 μg /mL) determined by the DCFH-DA probe,Annexin FITC-V/PI double staining method,and JC-1 probe. RESULTS The IC_(50) of PC12 cells was 1...