期刊论文

Zhishi Tongjiang Granule mediated control of MK2 phosphorylation in inhibiting postoperative ileus responsible for lung injury

中文

中成药

1001-1528

2021

43

08

2041-2047

10.3969/j.issn.1001-1528.2021.08.012

湖南省中医药管理局重点研究室-中医药防治肛肠疾病重点研究室项目（湘中医药函[2020]51）； 长沙市自然科学基金项目（kq2014217）； 湖南中医药大学中医学国内一流建设学科开放基金（2018ZYX18）;湖南中医药大学研究生科研创新课题（2018CX17）；

本校为第一机构

目的研究枳实通降颗粒对P38通路中MK2磷酸化及术后炎性肠梗阻(POI)相关性肺损伤的影响,探讨其作用机制。方法先将84只大鼠随机分为空白组,假手术组,模型组,枳实通降颗粒低、中、高剂量组(4.2、8.3、 16.6 g /kg)和普芦卡必利组(0.18 mg /kg) 。造模术后6 h,所有大鼠均按5 mL/kg给药量一次性灌胃给药。药物干预组予相应药物,其余各组予同等量生理盐水。术后24 h取材,检测各组大鼠肺组织中炎症因子IL-6、IL-8、TNF-α水平的变化及髓过氧化酶(MPO)的活性改变,HE染色观察肺组织的病理形态学改变、Western-blot及PCR分别检测肺组织中MK2 /p-MK2蛋白及mRNA的表达情况,并检测支气管肺泡灌洗液(BALF)中炎...

AIM To study the effects of Zhishi Tongjiang Granule on the phosphorylation of MK2 in p38 pathway and the lung injury associated with postoperative ileus (POI). METHODS Eighty-four rats were randomly divided into blank group,sham operation group,model group,Zhishi Tongjiang Granule groups for lowdose, medium-dose and high-dose uses (4.2,8.3,16.6 g /kg),and prucapride group (0.18 mg /kg). At 6 hours after modeling,all rats were given the corresponding drug (5 mL /kg) or same volume of normal saline by gavage. Twenty-four hours after the operatio...