期刊论文

中文

武警后勤学院学报(医学版)

2095-3720

2012

21

3

161-163,166

10.3969/j.issn.2095-3720.2012.03.003

本校为第一机构

【目的】探讨肝癌患者血清端粒酶逆转录酶（human telomerase reverse transcriptase,hTERT）mRNA表达水平及对肝癌的临床应用价值。【方法】设计人端粒酶逆转录酶基因引物和实时荧光PCR相对定量法反应体系,在ABI7500实时荧光PCR仪上检测45例肝癌患者,52例病毒性肝炎患者,29例肝硬化患者和58例健康人血清端粒酶逆转录酶mRNA表达水平并分析其差异。【结果】肝癌患者血清hTERT mRNA水平明显高于肝炎、肝硬化患者及健康人。肝癌患者血清hTERT mRNA相对表达量与肿瘤大小和分化等级及分期明显相关,与年龄、性别、以及肿瘤数量无明显关联。【结论】肝癌患者血清hTERT基因表达上调,其升高程度与肿瘤分化...

【Objective】 To investigate the expression level of hTERT in serum of hepatic cancer patients and its clinical significance.【Methods】 The mRNA of hTERT in serum were detected by real-time relative quantitative PCR method in 45 patients with hepatic cancer,52 patients with virus hepatitis,29 patients with hepatic cirrhosis and 58 healthy individuals.【Results】 The expression level of hTERT in serum of hepatic cancer group was significantly higher than other groups.The relative expression level of hTERT mRNA was demonstrated to be definitely ...