[Tao, Xueqing; Liu, Bin; Tong, Chunyi; Hu, Yalei] Hunan Univ, Coll Biol, Hunan Prov Key Lab Plant Funct Genom & Dev Regula, Changsha 410082, Hunan, Peoples R China.
[Wang, Wei; Xie, Qian] Hunan Univ Chinese Med, Sch Pharm, Innovat Mat Med Res Inst, TCM & Ethnomed Innovat & Dev Int Lab, Changsha 410208, Peoples R China.
[Chang, Li] Chinese Acad Agr Sci, Inst Bast Fiber Crops, Changsha 410008, Peoples R China.
Natural Science Foundation of Hunan ProvinceNatural Science Foundation of Hunan Province [2021JJ3016]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81673179, 31672457]; National Standardization Project of Traditional Chinese Medicine [ZYBZH-Y-HUN-23]
机构署名：
本校为其他机构
院系归属：
药学院
摘要：
Ribonuclease H is essential for the research and development of complex pathema. The high rigidity and versatility of DNA tetrahedrons means they are often used in biosensing systems. Inspired by "radar" technology, we proposed a radar-like monitor to detect RNase H activity in vitro and in situ by integrating DNA tetrahedral elements. The structure of a radar-like monitor was self-assembled from five customized single nucleic acid strands. Four DNA strands were assembled as DNA tetrahedrons with a long strand labeled by Dabcyl (quencher) at one of the apexes, while the fifth strand (DNA-RNA h...
