期刊论文

中文

中华实用诊断与治疗杂志

1674-3474

2018

32

3

218-221

10.13507/j.issn.1674-3474.2018.03.004

广西自然科学基金（2014GXNSFBA118192）；

本校为第一机构

目的 探讨PI3K/Akt信号通路在人结肠癌HT-29细胞增殖中的作用.方法 人结肠癌HT-29细胞分为结肠癌组和抑制剂组,癌旁正常细胞株为对照组,均取对数生长期细胞进行实验.结肠癌组和对照组应用培养基+等量生理盐水进行培养,抑制剂组应用培养基+ PI3K/Akt信号通抑制剂LY294002 10 μL进行培养.MTT法检测3组培养24、48、72 h时细胞增殖情况,Western blot法检测3组PI3K、Akt、磷酸化Akt、P-糖蛋白表达,流式细胞仪检测3组细胞周期及凋亡情况.结果 培养24、48、72 h,对照组和抑制剂组细胞吸光度值均小于结肠癌组(P＜0.05),对照组与抑制剂组比较差异无统计学意义(P≥0.05);培养24 h,对照组和抑制剂组PI3K、...

Objective To investigate the influence of PI3K/Akt signaling pathway on the proliferation of HT-29 human colon cancer cells.Methods The HT-29 cells were divided into cancer group and inhibitor group,and the adjacent normal cells were as controls (control group).The cells in logarithmic growth phase were tested.Inhibitor group was interfered by 10 μL of PI3K/Akt signaling inhibitor LY294002 + culture medium.Cancer group and control group were interfered by 10 μL of normal saline + culture medium.MTT assay was used to detect the proliferation o...