期刊论文

中文

湖南中医药大学学报

1674-070X

2007

27

1

5-8

10.3969/j.issn.1674-070X.2007.01.002

湖南省自然科学基金资助项目（06JJ50067）； 湖南省教育厅科学研究（青年）项目（A0141）；

本校为第一机构

目的 探讨补肾活血方对恒河猴多囊卵巢(PCO)模型卵巢局部环氧化酶-1(COX-1mRNA)表达的影响.方法 选择健康育龄雌性恒河猴(6只)随机分为正常对照组、模型对照组、补肾活血方组;采用皮下注射丙酸睾丸酮(T)和绒毛膜促性腺激素(HCG)建立PCO模型,分别灌胃给予生理盐水、补肾活血方,采用原位杂交技术观察各组卵巢局部COX-1mRNA表达的变化.结果 各组卵巢组织均可见COX-1基因表达的信号,以颗粒细胞表达最丰富;其中模型组卵泡膜细胞呈现异常高表达;补肾活血方组表达水平接近正常对照组.结论 恒河猴PCO模型卵巢局部COX-1mRNA异常表达,可能与多囊卵巢综合征(PCOS)发病有关;补肾活血方有可能通过降低COX-1mR...

Objective To explore the effect of Bushen Huoxue formula（BHF） on the expression of cyclooxygenase-1（COX-1） mRNA in the rhesus polycystic ovary（PCO）. Methods Six adult female rhesus monkeys were randomly divided into three .groups as control group, model group and BHF group; the PCO rhesus animal models were established by subcutaneous injection of testosterone and HCG, and filling stomach with normal saline and BHF respectively; then the expression changes of COX-1 mRNA in the ovary tissue were detected with in situ hybridization （ISH）e...