期刊论文

中文

中国临床药理学与治疗学

1009-2501

2016

21

8

873-877

31371161:国家自然科学基金 81673722:国家自然科学基金 14JJ1024:湖南省自然科学基金 2015JJ2117:湖南省自然科学基金

本校为第一机构

目的:探讨竹节参皂苷IVa甲酯(chikusetsu saponin iva methyl,CSIM)对血管紧张素II(angiotensin,Ang-II)刺激的血管平滑肌细胞(vascular smooth muscle cells,VSMCs)增殖的影响及机制研究。方法:以大鼠血管平滑肌细胞为实验对象,建立Ang-II刺激的VSMCs增殖模型。MTT法检测CSIM对VSMCs的毒性; CCK-8法、BrdU法检测CSIM对VSMCs增殖的影响,划痕实验检测 CSIM对VSMCs迁移力的影响,免疫蛋白印迹法检测PTEN、NF-κB蛋白表达水平。结果: 3 μmol /L的CSIM可显著抑制Ang-II诱导的 VSMCs增殖(P < 0. 05)与迁移(P < 0. 05),并伴随着PTEN蛋白的明显上调(P < 0. 05)及NF-κB蛋白表达的显著性降低(P < 0. 05)...

AIM: To observe the effects and mechanism of chikusetsu saponin iva methyl to vascular smooth muscle cells (VSMCs) proliferation. METHODS: VSMCs was cultured in vitro and the cell proliferation model was established by Ang-II. The VSMCs toxicity were determined by MTT assay,the proliferation of VSMCs was detected by CCK-8 and BrdU assay,the VSMCs migration distance was detected by wound-healing assay,the PTEN,NF-κB protein expression was determined by Western blot assay. RESULTS: 3 μmol /L of chikusetsu saponin iva methyl significantly inhibi...