期刊论文

中文

中医杂志

1001-1668

2013

54

11

949-952

10.13288/j.11-2166/r.2013.11.005

国家自然科学基金资助项目（30973717）；

本校为第一机构

目的探讨冠心病血瘀证差异表达基因及其启动子甲基化的情况。方法以家系冠心病血瘀证患者14例为观察对象,家系健康人7例为对照,采用表达谱芯片检测冠心病血瘀证差异表达基因。再收集冠心病血瘀证患者16例和健康人7例,选择其中2个差异基因进行启动子区甲基化特异性PCR(MS-PCR)研究,初步分析冠心病血瘀证差异表达基因及其启动子甲基化状态。结果表达谱芯片结果通过倍数变化值(FC)>3或FC0.05)。结论冠心病血瘀证差异基因KLF5和LRP12的启动子甲基化状态和健康人比较无明显区别。

Objective To research the differentially expressed gene and promoter methylation status of coronary heart disease（CHD）with blood stasis pattern.Methods Fourteen family-related CHD patients with blood stasis pattern were selected as observation subjects and 7healthy persons were chosen as control.The differentially expressed genes of CHD with blood stasis pattern were detected by microarray.Another 16CHD patients with blood stasis pattern and 7healthy persons were chosen and 2differentially expressed genes were selected to conduct methylation-...