期刊论文

Proteomics study of the small intestinal epithelia of splenic asthenia syndrome mice and the adjustive action of dry radix ginseng

中文

中国中西医结合消化杂志

1671-038X

2008

16

6

351-354

湖南省科技厅重点项目（04SK007-14）；

本校为第一机构

[目的]研究脾虚型小鼠小肠上皮细胞蛋白质组特征及白参的调节作用。[方法]昆明种小鼠60只，利血平法复制动物模型，随机分为正常、脾虚模型和自参汤剂组，双向凝胶电泳技术分离蛋白质，改进的考染法染色，以PDQuest软件分析图谱，基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）进行质谱分析。[结果]鉴定出的33个蛋白质点中有27个表达明显减弱，6个表达增强。经白参汤剂治疗后，其表达多数得以回调。各蛋白质的功能类型几乎涉及小肠细胞各主要功能。[结论]脾虚型小鼠小肠上皮细胞的蛋白质组特征主要表现为蛋白质表达呈双向变化，以下调为主。表达变化的蛋白质涉及细胞各部位，白参汤剂对小肠...

[Objective]To approach the character of proteomics of small intestinal epithelia of the splenic asthenia syndrome mice and the adjustive action of dry Radix Ginseng. [Methods]Sixty Kunming mice were divided randomly into 3 groups： normal group, model group and dry Radix Ginseng decoction group. Splenic asthenia syndrome animal model were duplicated by reserpine. Two dimensional polyacrylamide gel electrophoresis technology was applied to separate proteins of small intestinal epithelia of mice of every group. The proteins spots were visualized ...