期刊论文

中文

中国中西医结合消化杂志

1671-038X

2012

20

3

97-101

10.3969/j.issn.1671-038X.2012.03.001

湖南省教育厅科学研究资助项目（08c619） 国家自然科学基金资助项目（81072779） 湖南省普通高等学校青年骨干教师培养基金

本校为第一机构

[目的]比较健脾法、补肾法和健脾补肾法对慢性乙型肝炎（CHB）患者外周血树突状细胞（DCs）功能的影响。[方法]收集24例CHB患者和10例健康人的抗凝外周静脉血，分离外周血单个核细胞（PBMCs），进一步分离提取培养DCs，采用大鼠健脾、补肾和健脾补肾药物血浆进行干预。检测DCs细胞表面分子CDIα、CD80、CD86及藻红蛋白（HLA-DR）分子的表达。[结果]CHB组DCs表面分子CDIα、CD80、CD86及H1。A-DR的表达较健康对照组低（P〈0．05）。3组药物血浆干预后上述I）Cs表面分子表达率均有升高。其中，补肾药物血浆干预后DCs表面分子CDIα、CD80、CD86、HI-A-DR表达率较干预前均有明显提高（P〈0．05），且...

[Objective]To investigate the effect of different treatments of TCM（tonifying kidney, strengthening spleen and tonifying kidney combined with strengthening spleen）on DC cells in patients with chronic hepatitis B（CHB）. [Methods]Twenty-four patients with chronic hepatitis and ten healthy controls were included in this study. DC cells of phenotype CDIα, CDSO, CD86 and HLA-DR were differentiated from peripheral blood mononuclear cells （Pt3MCs）, incubated and induced. Then the DC cells were interfered by plasma of medicine of different treatm...