期刊论文

Impact of Ureaplasma urealyticum infection on the MRPS22 protein expression in rat spermatogenic cells and intervening effect of Zhibai Dihuang Decoction

中文

中华男科学杂志

1009-3591

2019

25

1

55-61

10.13263/j.cnki.nja.2019.01.010

81774324:国家自然科学基金 81373641:国家自然科学基金 81603634:国家自然科学基金 2013161:湖南省中医药局项目 2016-39:湖南省大学生研究性学习和创新性实验计划 2017-3:湖南省大学生研究性学习和创新性实验计划

本校为第一机构

目的:观察解脲支原体（UU）感染对大鼠睾丸生精细胞线粒体核糖体蛋白S22（MRPS22）表达的影响及知柏地黄汤的干预作用。方法:UU感染SD雄性大鼠模型45只,随机分成模型组、知柏地黄汤组、西药组,并以健康大鼠为对照组,每组15只。造模成功后第10天起知柏地黄汤组予以知柏地黄汤灌胃[1 g/（k·d）],西药组予以阿奇霉素灌胃[0.105 g/（k·d）],对照组、模型组予以同体积生理盐水灌胃,连续灌胃21 d。采用TUNEL法检测生精细胞凋亡,电镜观察生精细胞线粒体结构,流式细胞仪检测线粒体膜电位（MMP）,RT-PCR检测大鼠睾丸生精细胞MRPS22 m RNA表达,Western印迹检测大鼠睾丸生精细胞MRPS22蛋白表达。观察各组...

Objective:To investigate the impact of Ureaplasma urealyticum (UU) infection (UUI) on the expression of the mitochondrial ribosomal protein S22 (MRPS22) in rat spermatogenic cells and the intervening effect of Zhibai Dihuang Decoction (ZBDH).Methods:Forty-five SD male rats were randomly divided into four groups of equal number:normal control,UUI model control,ZBDH and azithromycin,and the UUI model was made by bladder injection of the standard UU strain in the latter three groups.After modeling,the rats in the ZBDH and azithromycin groups were ...