期刊论文

Xiongcan Yishen Prescription up-regulates the expressions of cholesterol transport proteins,steroidogenic enzymes and SF-1 in the Leydig cells of rats with late-onset hypogonadism

中文

中华男科学杂志

1009-3591

2020

26

03

258-264

10.13263/j.cnki.nja.2020.03.013

国家自然科学基金（81202706、81673984）； 湖南省自然科学基金（2018JJ3402）； 长沙市科技计划项目经费资助-长沙市杰出创新青年人才项目（kq1802015）； 湖南省***卫生人才225工程医学学科骨干人才培养对象（湘卫函[2019]196号）； 湖南创新型省份建设专项经费资助-“湖湘青年英才”支持计划（2019RS2041）； 中华中医药学会“青年人才托举工程”（2019-QNRC2-C07）~~；

本校为第一机构

目的:探讨雄蚕益肾方对LOH大鼠Leydig细胞胆固醇转运蛋白、睾酮合成酶和SF-1表达的影响.方法:25只18月龄雄性SD大鼠随机均分为模型组、丙酸睾酮组、雄蚕益肾方-低、中、高剂量组,2月龄雄性SD大鼠5只设为正常组.雄蚕益肾方-低、中、高剂量组分别给予10.4、20.8、41.6 g/(kg·d)体重剂量的雄蚕益肾方配方颗粒剂灌胃;丙酸睾酮组大鼠肌注5.21 mg/(kg·d).体重剂量的丙酸睾酮,每周3次;正常组和模型组给予相同体积的蒸馏水灌胃,均连续28 d.实验结束后取睾丸组织进行Western印迹检测StAR、TSPO、CYP11 A1、HSD3B7、HSD17B4和SF-1蛋白表达. 结果:模型组大鼠睾丸组织StAR、TSPO、CYP11A1、HSD3B7、HSD17B...

Objective: To investigate the effects of Xiongcan Yishen Prescription (XYP) on the expressions of cholesterol transport proteins,steroidogenic enzymes and steroidogenic factor-1 (SF-1) in the Leydig cells of the rats with late-onset hypogonadism (LOH). Methods: Twenty-five 18-month-old male SD rats were randomly divided into five groups of equal number,LOH model control,testosterone propionate (TP) and low-,medium-and high-dose XYP,and another 5 two-month-old male SD rats included as normal controls. After modeling,the animals in the TP group were treated by intramuscular injection of TP at 5....