期刊论文

Xiongcanyishen Prescription increases mitochondrial autophagy of Leydig cells in LOH rats via PINK1 and Parkin pathways

中文

中华男科学杂志

1009-3591

2020

26

12

1129-1134

10.13263/j.cnki.nja.2020.12.013

国家自然科学基金（81673984,82074444）； 湖南省教育厅重点项目（20A368）； 长沙市科技计划项目经费资助-长沙市杰出创新青年人才项目（kq1802015）； 湖南省***卫生人才225工程医学学科骨干人才培养对象（湘卫函[2019]196号）； 湖南创新型省份建设专项经费资助-“湖湘青年英才”支持计划（2019RS2041）； 中华中医药学会“青年人才托举工程”（2019-QNRC2-C07）~~；

本校为第一机构

目的:探讨雄蚕益肾方介导PINK1/Parkin通路对迟发性性腺功能减退症(LOH)大鼠睾丸间质细胞(LC)线粒体自噬的影响.方法:20只18月龄雄性SD大鼠随机均分为LOH模型组,雄蚕益肾方低、中、高剂量组,2月龄雄性SD大鼠5只设为正常对照组.雄蚕益肾方低、中、高剂量组分别给予10.4、20.8、41.6 g/kg体重剂量的雄蚕益肾方配方颗粒剂灌胃;正常对照组和LOH模型组给予相同体积的蒸馏水灌胃,均连续28 d.实验结束后电镜观察睾丸组织LC超微结构,Western印迹检测PINK1、Parkin和p62蛋白表达.结果:正常对照组LC线粒体形态基本正常,存在明显自噬现象,LOH模型组线粒体自噬少,雄蚕益肾方低、中、高剂量组线粒体有自噬发生...

Objective: To investigate the effects of PINK1 and Parkin pathways mediated by Xiongcanyishen Prescription (XP) on the mitochondrial autophagy of Leydig cells in male rats with late-onset hypogonadism (LOH).Methods: Twenty 18-month-old male SD rats were randomly divided into an LOH model control, a low-dose XP, a medium-dose XP and a high-dose XP group, and another 5 two-month-old male SD rats were included as normal controls. The animals in the low-, medium- and high-dose XP groups were treated intragastrically with XP granules at 10.4, 20.8 and 41.6 g/kg, while the normal and LOH model contr...