期刊论文

中文

中国中医药信息杂志

1005-5304

2017

24

12

42-46

10.3969/j.issn.1005-5304.2017.12.011

国家自然科学基金

本校为第一机构

目的观察甘露消毒丹及其拆方对柯萨奇病毒A16型(CoxA16)小鼠模型P-选凝素糖蛋白配体1(PSGL-1)和促炎因子的影响,探讨其抗病毒的作用机制。方法7日龄ICR小鼠150只,随机分为正常组、模型组、全方组、芳残方组、清残方组和利残方组,每组25只。除正常组外,其余各组小鼠腹腔注射20 μL 10~7TCID_(50)的CoxA16标准株原液进行造模。除正常组和模型组外,其余各组给予相应药物干预。于给药后第10日取材,检测PSGL-1、肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-1β、IL-4的表达,并进行病理组织观察。结果除正常组外,其余各组小鼠肌肉组织内存在大量CoxA16,表明造模成功;HE染色结果显示,甘露消毒丹及其拆方可...

Objective To investigate the effects of Ganlu Xiaodu Pills and its residues on PSGL-1 and proinflammatory cytokines in Coxsackie virus A16 (CoxA16) mouse model; To discuss its antiviral mechanism of action. Methods Totally 150 ICR mice at age of 7 days were randomly divided into normal group, model group, all-side group, aromatic residual group, clearing residual group and removing residual group, with 25 mice in each group. Except for normal group, other groups were injected intraperitoneally with 20 μL of 10~7TCID_(50) CoxA16 standard stock ...