期刊论文

Effects of Kelong Capsule on Benign Prostatic Hyperplasia Rat Model and Nrf2/ARE Signaling Pathway

中文

中国实验方剂学杂志

1005-9903

2018

24

11

87-91

10.13422/j.cnki.syfjx.20181127

国家自然科学基金面上项目（81373708） 湖南中医药大学中医诊断学国家重点学科开放基金项目（2014-4）

本校为第一机构

目的:探讨克癃胶囊对丙酸睾酮诱导的大鼠前列腺增生模型的影响及抗氧化作用机制。方法:将40只雄性SD大鼠随机分为正常组,模型组,克癃胶囊低、高剂量组(3.6,7.2g·kg~(-1)),核因子E2相关因子2(Nrf2)干预剂组,每组8只。除正常组外,其余各组均皮下注射丙酸睾酮建立前列腺增生的大鼠模型,同时给予药物灌服,连续4周。观察不同药物对前列腺湿重、前列腺指数的影响,苏木素-伊红(HE)观察病理组织形态变化,酶联免疫吸附测定法(ELISA)检测血清样品中丙二醛(MDA)和谷胱甘肽(GSH)的含量,实时荧光定量聚合酶链式反应(Real-time PCR)检测前列腺组织中Nrf2/ARE信号通路抗氧化因子Nrf2,血细素单加氧酶-1(HO-1),醌...

Objective:To investigate the effect of Kelong capsule on testosterone-induced benign prostatic hyperplasia (BPH) and its antioxidant mechanism in rats.Method:Forty male SD rats were randomly divided into normal group,model group,low-dose Kelong capsule group (3.6 g·kg~(-1)),high-dose Kelong capsule group (7.2 g·kg~(-1)) and nuclear factor E2-related factor 2 (Nrf2) intervention group,n = 8 in each group.Except the rats in normal group,all the other rats received subcutaneous injections of testosterone propionate (TP) to establish BPH models.D...