期刊论文

中文

现代生物医学进展

1673-6273

2010

10

17

3209-3211,3234

10.13241/j.cnki.pmb.2010.17.045

30971170:国家自然科学基金 2008XQD02:南华大学院士基金

本校为其他机构

目的:观察Bmi-1稳定干扰后对乳腺癌细胞药敏性的影响.方法:构建Bmi-1 shRNA逆转录病毒表达载体,并用该逆转录病毒感染人乳腺癌细胞,建立Bmi-1稳定干扰的乳腺癌细胞株.采用不同浓度的化疗药物5-氟尿嘧啶和顺铂处理上述细胞,MTT法观察乳腺癌细胞的生长抑制率.结果:免疫印迹检测结果表明Bmi-1稳定干扰的乳腺癌细胞株成功建立.MTT检测显示Bmi-1稳定干扰的乳腺癌细胞(MDA-MB-435S-Bmi-1/RNAi)增殖抑制率显著高于对照细胞(MDA-MB-435S-vector)(P

Objective：To investigate the effect of Bmi-1 knockdown on the chemosensitivity of breast cancer cells.Methods：pSUPER Retro Bmi-1 RNAi plasmid was constructed successfully.Breast cancer cells were infected with the viral supernatant from the GPG293 cells.The alteration of Bmi-1 expression was examined by Western blot assay.Breast cancer cells were treated with different concentrations of 5-fluorouracil and cisplatin,and the effect of Bmi-1 knockdown on the proliferation of breast cancer cells treated with 5-fluorouracil and cisplatin were meas...