期刊论文

Effects of CXCL8 on the Development of Atherosclerosis in Mice and Its Molecular Mechanism Study

中文

现代生物医学进展

1673-6273

2020

20

6

1057-1060

10.13241/j.cnki.pmb.2020.06.012

教育部重点实验室开放基金项目（ZYNK201702）；

本校为第一机构

目的:探讨趋化因子8（CXCL8）对小鼠动脉粥样硬化（AS）发生发展的影响及其可能机制。方法:40只雄性Apo E-/-小鼠随机分为AS组和CXCL8抑制剂（G31P）组,每组各20只,另选取20只雄性C57BL/6J小鼠作为对照组。AS组小鼠予高脂饲料喂养12周,G31P组小鼠予高脂饲料喂养12周的同时皮下注射G31P（注射剂量为500μg/kg）,对照组的小鼠予普通饲料喂养12周。ELISA法检测小鼠血清中的甘油三脂（TG）、低密度脂蛋胆固醇（LDL-C）、高密度脂蛋白胆固醇（HDL-C）、总胆固醇（TC）水平,实时聚合酶链反应（RT-PCR）法检测小鼠主动脉组织中CXCL8及基质金属蛋白酶9（MMP9）mRNA的表达量,Western-blot法检测主动脉CXC...

Objective: To investigate the effect of chemokine 8(CXCL8) on the development of atherosclerosis(AS) in mice and its possible mechanism. Methods: 40 male Apo E-/-mice were randomly divided into AS group and G31 P group, 20 mice in each group,meanwhile 20 male C57 BL/6 J mice was assigned into the control group. AS group mice were fed with high fat diet for 12 weeks, G31 P group mice were were fed with high fat diet for 12 weeks and subcutaneous injection at the same time of G31 P(The injection volume is500 μg/kg), The control group mice were f...