期刊论文

中文

中国民族民间医药

1007-8517

2020

29

6

14-18

10.3969/j.issn.1007-8517.2020.6.zgmzmjyyzz202006006

湖南省科技厅重点研发项目(2017SK2124) 湖南省一流学科中药学校行科字[2018]3号。

本校为其他机构

目的:建立人工栽培黄花石蒜乙醇提取物HPLC指纹图谱。方法:黄花石蒜乙醇提取物的分析采用ZORBAX Eclipse XDB-C18色谱柱（4. 6×250 mm,5μm）;流动相:甲醇（A）-水（含0. 1%磷酸,B）,梯度洗脱见表2;柱温:30℃;紫外检测波长:238 nm;流速:1. 0 m L/min。结果:以加兰他敏为参照峰,确定了13个特征峰,10批样品指纹图谱相似度均在0. 9以上。结论:此方法操作简单,准确可靠,可为黄花石蒜鉴别及内在质量评价提供参考依据。

Objective To establish the HPLC fingerprints of the artificial cultivation Lycoris aurea(L’Her.)Herb.ethanol extract.Methods The analysis of Lycoris aurea(L’Her.)Herb.ethanol extract was developed on a 30℃ thermostatic ZORBAX Eclipse XDB-C18(4.6×250 mm,5μm),with the mobile phase comprising of Methanol-0.3% phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 238 nm.Results It was determined 13 characteristic peaks with galantamin as reference material,which has.The HPLC fingerprints of ...