期刊论文

中文

疑难病杂志

1671-6450

2018

17

6

585-588

10.3969/j.issn.1671-6450.2018.06.011

本校为第一机构

目的 研究ABCC4在胃癌组织中的表达情况及其对化疗药物耐药性的影响.方法 2011年1月—2013年12月湖南中医药大学第一附属医院外科诊治胃癌患者115例,采用免疫组织化学方法检测ABCC4基因的表达;取部分切除的胃癌组织样本,培养待检测细胞,在体外用MTT比色法检测细胞对抗癌药物(顺铂和5-氟尿嘧啶)的敏感性,随访并分析不同ABCC4基因表达组耐药性、胃癌复发率、复发时间的差异.结果 ABCC4基因在胃癌组织中的阳性表达率为46.96%(54/115),MTT比色法检测表明,阳性表达组胃癌组织对顺铂和5-氟尿嘧啶的敏感度显著低于阴性表达组(t=9.14、10.49,P0.05),但复发时间阳性表达组早于阴性表达组(P0.05).结论 ABC...

Objective To explore the expression of ATP binding cassette, subfamily C member 4 (ABCC4) in gas-tric cancer tissue and the effect of this gene on chemotherapeutic drug resistance of gastric cancer. Methods The expression of ABCC4 gene was detected by immunohistochemical method in 115 patients with gastric cancer who were treated at surgery department in the first hospital of Hunan university of Traditional Chinese Medicine between Janurary 2011 to December 2013. The cell culture of gastric cancer tissue was performed, and the sensitivity of the patient' s tumor cells to anti-cancer drugs ( Ci...