期刊论文

中文

中华男科学杂志

1009-3591

2015

21

3

256-262

10.13263/j.cnki.nja.2015.03.012

国家自然科学基金项目资助（81173264） 湖南省教育厅重点项目资助（11A088） 国家中医药管理局中医男科重点学科资助 湖南湖南省教育厅中医外科重点学科资助

本校为第一机构

目的: 观察麝香、乳香含药血浆对前列腺抗原刺激诱导的单核细胞炎症因子释放及前列腺细胞或内皮细胞炎症反应相关蛋白表达的影响。 方法: 采用SD大鼠制备麝香、乳香含药血浆,BALB/c小鼠制备单核细胞与前列腺抗原。单核细胞以梯度浓度(0,2.5%,5%,10%,20%)的麝香、乳香含药血浆预处理1 h后,前列腺抗原诱导活化,培养96 h时ELISA法检测炎症因子释放水平;前列腺细胞RWPE-1与内皮细胞EA.hy926共培养,梯度浓度DP-M&O预处理1 h后,加入经PAgs诱导活化的单核细胞,培养96 h时Western印迹法检测RWPE-1或EA.hy926细胞炎症反应相关蛋白表达水平。 结果: 麝香、乳香含药血浆随浓度增加,降低TNF-α、IL-1β、IL-...

OBJECTIVE: To study the effects of drug plasma of musk and olibanum (DP-M&O) on the release of inflammatory cytokines from monocytes and the expressions of the proteins associated with inflammation of prostatic or endothelial cells induced by prostate antigen (PAg) stimulation. METHODS: We prepared DP-M&O using SD rats and monocytes and PAgs using BALB/c mice. We pre-treated the monocytes with DP-M&O at the gradient concentrations of 0, 2.5, 5, 10, and 20% for 1 hour, activated them with PAgs, and then cultured them for 96 hours, followed by detection of the release of inflammatory cytokines. ...