期刊论文

Cytoplasmic M-CSF Induces Invasion and Migration of HeLa Cells

Tang Sheng-Song

中文

生物化学与生物物理进展

1000-3282

2017

44

11

1007-1015

10.16476/j.pibb.2017.0045

Hunan School of Higher Learning Foundation of Science and 266 Technology Innovation Platform [09K072, 12K120]; Youth Foundation of the Education Department of Hunan Province [11B110]

本校为第一机构

为探讨巨噬细胞集落刺激因子(M-CSF)对人宫颈癌细胞(HeLa细胞)侵袭和迁移的影响及机制,采用胞质定位空载体 pCMV/cyto/myc与重组载体pCMV/cyto/myc-M-CSF稳定转染HeLa细胞株,建立稳定高表达胞质M-CSF的细胞系(HeLa-M细胞).经Transwell实验观察胞质M-CSF对HeLa细胞侵袭和迁移能力的影响,逆转录-聚合酶链式反应及蛋白质印迹检测细胞Rho三磷酸鸟苷酶(Rho GTPases)及基质金属酶的表达,明胶酶谱法检测基质金属蛋白酶2的活性.结果显示,与转染空载体的HeLa细胞(HeLa-C细胞)和对照组HeLa细胞比较,胞质M-CSF的高表达可明显增强HeLa细胞在体外的侵袭和迁移能力,其机制与Rho GTPases的活化,以及MMP2表达上调...

In order to study the effects and mechanisms of macrophage colony stimulating factor (M-CSF) on invasion and migration in human cervical cancer cells (HeLa cell line), the empty vectors (pCMV/cyto/myc) and recombinant vectors (pCMV/cyto/myc-M-CSF) were transfected into HeLa cells to establish the stable cell line with high expression of cytoplasmic M-CSF (HeLa-M cells). Transwell tests were used to observe the effect of cytoplasmic M-CSF on the cell invasion and migration. MMP-2 activity was detected by gelatin zymography assay. The expression of mRNA and protein was measured by reverse transc...