期刊论文

中文

老年医学与保健

1008-8296

2019

25

3

355-357+406

10.3969/j.issn.1008-8296.2019.03.021

国家自然科学基金(81874462) 湖南省自然科学基金(2016JJ6084) 湖南省中医药管理局科学研究项目(201662) 湖南省科卫联合基金(2018JJ6107).

本校为第一机构

目的 析因设计探讨淫羊藿苷(ICA)和三七总皂苷(PNS)在抑制阿尔茨海默病(Alzheimer's disease,AD)模型PC12细胞损伤中是否具有协同增效作用.方法 用Ap25-35接触PC12细胞24h,诱导建立AD细胞模型,实验设空白组、模型组、阳性对照组、ICA组、PNS组和ICA+ PNS组,给予相应药物干预后,处理细胞,进行指标检测.MTT法检测细胞的存活率;流式细胞术检测细胞的凋亡情况.结果 空白组细胞未经Ap25-35损伤,细胞活性好,较少发生凋亡,经Ap25-35损伤的AD模型细胞,细胞活性明显下降,细胞凋亡率明显升高,与空白组比较,差异显著(P＜0.01).各药物干预组均能不同程度改善AD模型细胞的损伤情况,抑制细胞凋亡,与模型组比较...

Objective To explore the synergistic effect of icariin and panax notoginseng saponins (PAS) in inhibiting PC12 cell injury in Alzheimer's disease (AD) models with factorial design.Methods PC12 cells were exposed to Abeta 25-35 for 24 hours,and AD cell model was induced;blank group,model group,positive control group,ICA group,PNS group and ICA+PNS group were set up in the experiment;after corresponding drug interventions,the cells were treated and the indicators were detected;cell viability was detected by MTT assay while cell apoptosis was detected by flow cytometry. Results The AD model cells...