期刊论文

中文

中国细胞生物学学报

1674-7666

2022

44

6

982-990

10.11844/cjcb.2022.06.0002

本校为第一机构

为探讨吴茱萸碱(Evodiamine)介导miR-223-3p对缺氧诱导的心肌微血管内皮细胞增殖、迁移和血管生成的机制,该研究体外分离培养大鼠心肌微血管内皮细胞,将细胞分为对照组(Con),缺氧组(Hypoxia),吴茱萸碱低、中、高剂量组(Evodiamine-L、M、H), anti-miR-NC组, anti-miR-223-3p组,吴茱萸碱+miR-NC组(Evodiamine+miR-NC),吴茱萸碱+miR-223-3p组(Evodiamine+miR-223-3p),除Con组除外,其余各组均进行缺氧处理。采用MTT检测细胞增殖变化; Transwell实验检测细胞迁移能力; Western blot检测VEGF蛋白表达情况;血管生成实验检测血管形成情况; RT-qPCR检测miR-223-3p表达水平。结果显示, Hypoxia组的细胞活...

To investigate the mechanism of Evodiamine-mediated miR-223-3p on the proliferation,migration and angiogenesis of myocardial microvascular endothelial cells induced by hypoxia,rat myocardial microvascular endothelial cells were isolated and cultured in vitro in this study.The cells were divided into control group (Con),hypoxia group (Hypoxia),low,medium,and high dose evodiamine groups (Evodiamine-L,M,H),anti-miR-NC group,anti-miR-223-3p group,Evodiamine+miR-NC group (Evodiamine+miR-NC),Evodiamine+miR-223-3p group (Evodiamine+miR-223-3p).The other groups were all treated with hypoxia except for...