期刊论文

中文

中国实验方剂学杂志

1005-9903

2016

22

6

84-88

10.13422/j.cnki.syfjx.2016060084

81273990:国家自然科学基金 2013000011:高等学校博士学科点专项科研基金(博导类) 1300001:湖南省中医药局重点项目

本校为第一机构

目的:观察不同剂型七味白术散对肠道菌群失调模型小鼠小肠黏膜上皮白细胞介素-4(IL-4),IL-10,干扰素-α(IFN-α) mRNA表达的影响。方法:采用盐酸林可霉素和头孢曲松钠注射液联合中药番泻叶ig小鼠制备肠道菌群失调腹泻模型。造模成功后,将小鼠随机分为模型组(MG)(随后称自然恢复组ZHG),七味白术散水煎液组(QJG,6 g·kg~(-1)),七味白术散滴剂组(QDG,1.36 g·kg~(-1)),七味白术散提取物组(QTG,0.27 g·kg~(-1)),并设正常组(NG)。MG(ZHG)与NG给予蒸馏水ig,其余各治疗组小鼠给予相应剂型药物ig。在造模第7,9,11,13天,各组各时间点取10只小鼠小肠行逆转录-聚合酶链式反应(RT-PCR)检测小鼠小肠黏膜上皮...

Objective:To investigate the effects of different formulated Qiwei Baizhu San on the mRNA expression of interleukin-4 (IL-4), IL-10 and interferon-α (IFN-α) in intestinal mucosal epithelia of mice with intestinal flora alteration. Method:Ceftriaxone, lincomycin hydrochloride and folium sennae were administered to mice by gavage to establish intestinal flora alteration type diarrhea models. After modeling, the mice were randomly divided into model group (MG, referred to as natural recovery group ZHG), Qiwei Baizhu decoction group (QJG, 6 g·kg...