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Purification and Identification of miRNA Target Sites in Genome Using DNA Affinity Precipitation

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成果类型:
期刊论文
作者:
Xun, Yu;Tang, Yinxing;Hu, Linming;Xiao, Hui;Long, Shengwen;...
通讯作者:
Wei, Ke;Xiang, SL;Wei, K
作者机构:
[Wei, Chenxi; Xiang, Shuanglin; Hu, Linming; Long, Shengwen; Wei, Ke; Xiao, Hui; Tang, Yinxing; Gong, Mengting; Xun, Yu] Hunan Normal Univ, Key Lab Prot Chem & Dev Biol Educ, Minist China, Coll Life Sci, Changsha, Hunan, Peoples R China.
[Wei, Ke] Hunan Univ Chinese Med, Med Sch, Changsha, Hunan, Peoples R China.
通讯机构:
[Xiang, SL ; Wei, K ; Wei, K] H
Hunan Normal Univ, Key Lab Prot Chem & Dev Biol Educ, Minist China, Coll Life Sci, Changsha, Hunan, Peoples R China.
Hunan Univ Chinese Med, Med Sch, Changsha, Hunan, Peoples R China.
语种:
英文
关键词:
DNA;affinity precipitation;genome;miRNA;target sites
期刊:
Frontiers in Genetics
ISSN:
1664-8021
年:
2019
卷:
10
页码:
456127
基金类别:
This research was funded by the China Natural Science Foundation (grant numbers 81770389, 81601122, and 81703919), Hunan Provincial Natural Science Foundation of China (grant number 2017JJ3205, 2017JJ3232), and Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province (grant number 20134486).
机构署名:
本校为其他机构
院系归属:
医学院
摘要:
Combination with genomic DNA is one of the important ways for microRNAs (miRNAs) to perform biological processes. However, because of lack of an experimental method, the identified genomic sites targeted by microRNA were only located in the promoter and enhancer regions. In this study, based on affinity purification of labeled biotin at the 3′-end of miRNAs, we established an efficiently experimental method to screen miRNA binding sequences in the whole genomic regions in vivo. Biotinylated miR-373 was used to test our approach in MCF-7 cells,...

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