期刊论文、会议论文

中文

中华中医药杂志

1673-1727

2014

29

5

1448-1453

第五届全国中医药博士生学术论坛

第五届全国中医药博士生学术论坛论文集

2014-04-19

长沙

中华中医药学会<&wdkj&>《中华中医药杂志》<&wdkj&>北京岐黄中医药文化发展基金会

国家自然科学基金(No.81173264);中国博士后基金(No.20110491256);湖南省教育厅重点资助项目(No.11A088)~~

本校为第一机构

目的:应用RWPE-1细胞单层模型体外模拟人体前列腺上皮屏障,分析探讨芳香开窍药物麝香配伍乳香对前列腺上皮屏障的促通透作用。方法:利用人前列腺上皮细胞RWPE-1制备细胞单层模型,以梯度浓度(2.5%, 5.0%, 10.0%, 20.0%)麝香配伍乳香含药血浆(DP-M&O)处理后检测细胞单层电阻TEER值与细胞毒性,采用酚红透过实验观察RWPE-1细胞单层细胞连接和通透性变化,并采用免疫荧光与Western Blot方法检测与细胞紧密连接功能相关的ZO-1蛋白表达情况。结果:经DP-M&O处理后,细胞单层电阻TEER值降低,且呈现明显时效关系,以30-60min时间段及10.0%,20.0%浓度时下降最明显;细胞毒性实验表明,2.5%、5.0%浓度DP-M&O 无细...

Objective： To investigate the the effects of resuscitation-inducing aromatic herbs of Musk and Olibanum to promote permeability on prostatic epithelial barrier with RWPE-1 cell monolayer model. Methods： The RWPE-1 cells were used to prepare as a cell monolayer model which had the function of epithelial tight junction like the human prostatic epithelial barrier in vitro, and treated with increasing dilution （2.5%, 5.0%, 10.0%, 20.0%） of drug plasma of Musk and Olibanum （DP-M＆O）. Thus the value of cell resistance TEER and cytotoxicity were...