期刊论文

Zhang, G.-M.

中文

中国组织工程研究

2095-4344

2015

19

1

78-84

10.3969/j.issn.2095-4344.2015.01.014

(81303123):国家自然科学基金资助项目 (2013T60770，2012M511382):中国博士后基金项目 (20114323120007):高等学校博士学科点专项科研基金项目 (2014XNFZ06):湖南省重点实验室开放基金 No.81303123:the National Natural Science Foundation of China, No.2013T60770,2012M511382:China Postdoctoral Science Foundation, No.20114323120007:Specialized Research Fund for the Doctoral Program of Higher Education, No.2014XNFZ06:the Hunan Provincial Key Laboratory Foundation,

本校为第一且通讯机构

背景：现有研究表明，人与大鼠卵巢优势卵泡中的颗粒细胞具有表达干细胞特性的现象。目的：探讨干细胞相关因子在大鼠卵巢颗粒细胞中的表达。方法：将大鼠卵巢组织制作石蜡切片后，使用免疫组化法检测CD34，CD133，ABCG2/Bcrp1，Pou5f1/Oct-4的表达。卵泡穿刺法获取颗粒细胞，并使用免疫组化法检测FSHR受体的表达以鉴定颗粒细胞的纯度。免疫组化法检测颗粒细胞 CD44， C-Kit 的表达情况， RT-PCR 检测卵巢组织与颗粒细胞 ABCG2/Bcrp1、Pou5f1/Oct-4、Nanog基因的表达情况。结果与结论：免疫组化法石蜡切片显示部分卵巢颗粒细胞CD34，CD133，ABCG2/Bcrp1，Pou5f1/Oct-4阳性表达，而Pou5f1/Oct-4蛋...

BACKGROUND: Human and rat ovarian granulosa cells in dominant follicles have the phenomenon of expressing stem cell characteristics. OBJECTIVE: To investigate the expression of stem cell-related factors in rat ovarian granulosa cells. METHODS: After the paraffin sections of rat ovarian tissue, immunohistochemical method was used to detect CD34, CD133, ABCG2/Bcrp1, Pou5f1/Oct-4 expressions. Granulosa cells cultured in vitro were harvested by follicular puncture method, and then the immunohistochemical method was used to detect the expression of ...