期刊论文

Effects of Modified Danshen Decoction on SSAT Activity of Rats with Myocardial Ischemia/Reperfusion Injury

中文

中国中医药信息杂志

1005-5304

2017

24

7

62-65

10.3969/j.issn.1005-5304.2017.07.015

81373576:国家自然科学基金 81503536:国家自然科学基金 14B136:教育厅项目 14C0872:教育厅项目 201304:湖南省中医药管理局重点课题 2014SK3034:湖南省科技厅一般项目 2016CX28:创新基金

本校为第一机构

目的观察加味丹参饮对SD大鼠心肌缺血再灌注损伤(IRI)模型精脒/精胺乙酰转移酶(SSAT)/多胺通路的影响,探讨其抗IRI的机制。方法结扎大鼠冠状动脉左前降支30 min再灌注90 min建立大鼠心肌IRI模型。实验大鼠随机分为正常对照组、假手术组、模型组和加味丹参饮组,每组10只。TTC染色测定大鼠心肌梗死面积,ELISA检测大鼠心肌组织SSAT含量,RT-PCR和Western blot分别检测大鼠心肌组织SSAT mRNA和蛋白表达,HPLC检测大鼠心肌组织多胺(腐胺、精脒、精胺)含量。结果与假手术组比较,模型组大鼠心肌梗死面积、SSAT含量增加,SSAT mRNA和蛋白表达升高,多胺含量降低(P

Objective To observe the effects of modified Danshen Decoction on spermidine/spermine acetyltransferase (SSAT) /polyamine pathways of SD rats with IRI; To investigate its protective mechanism. Methods The model of IRI was established by ligating left anterior descending coronary artery for 30 min followed by reperfusion for 90 min. The SD rats were randomly divided into the control group, sham-operation group, model group and modified Danshen Decoction group, with 10 rats in each group. The myocardial infarction size was measured by using TTC staining. The contents of SSAT were measured by ELI...