期刊论文

The effect of unilateral olfactory function deprivation on the expression of NeuN and CB in piriform cortex of adult guinea pigs

中文

解剖学研究

1671-0770

2017

39

5

357-361

湖南省教育厅优秀青年课题(16B269) 益阳医学高等专科学校引进***人才科研启动费(2016-001) 益阳市科技局课题(2015JZ42)

本校为其他机构

目的 观察嗅觉功能剥夺后对成年豚鼠两侧梨状皮质神经元特异性核蛋白(NeuN)、维生素D依赖性钙结合蛋白(CB)表达的影响.方法 将20只豚鼠随机分成两组,通过电烙铁损伤左侧外周鼻孔区建立嗅觉功能剥夺模型,饲养3周和6周之后灌注取材,采用尼氏染色与免疫组织化学技术观察各个时间点豚鼠自身两侧梨状皮质尼氏体、NeuN和CB的表达情况.结果 3周组与6周组嗅觉未剥夺侧梨状皮质尼氏体的密度、CB+细胞密度均显著高于剥夺侧(P＜0.05);3周组与6周组嗅觉未剥夺侧梨状皮质NeuN+细胞密度低于剥夺侧,差异有统计学意义(P＜0.05).结论 嗅觉功能活动对成年豚鼠梨状皮质神经元的表达有重要作用.

Objective To explore the effect of unilateral olfactory function deprivation on the expression of Neuron-specific nuclear protein (NeuN),Calbindin (CB) in piriform cortex of adult guinea pigs.Methods 20 guinea pigs were randomly divided into two groups,lefi olfactory functional deprivation model was established through electric cautery injury at the peripheral nostrils and guinea pigs were sacrificed survived for 3 and 6 weeks.The expression of the nissle body in piriform cortex of the adult guinea pigs was detected with Nissle staining,Further...