期刊论文

中文

中华行为医学与脑科学杂志

1674-6554

2011

20

4

292-294

10.3760/cma.j.issn.1674-6554.2011.04.002

基金项目：国家重点基础研究“973”计划课题资助项目（2009CB523002）

本校为第一机构

目的探讨四磨汤对慢性应激小鼠胃肠运动及对下丘脑、脊髓、十二指肠血管活性肠肽 (VIP)的影响. 方法30只小鼠随机分为正常组、慢性应激组和四磨汤组; 采用不可预知的多种刺激制备慢性应激模型; 21d后检测动物胃内残留率和小肠排空率, 分别采用免疫组化法、半定量逆转录-聚合酶联反应(RT-PCR)法检测下丘脑、脊髓、十二指肠VIP的表达. 结果与正常组[(49.81±8.56)％; (51.02± 5.11)％]比较, 慢性应激能增加胃内残留率[(61.53±8.71)％; P

Objective To explore effect of Simotang on gastrointestinal motility and expression of vasoactive intestinal peptide（VIP） in the hypothalamus, spinal cord and duodenum of chronical stressed mice. Methods Mice were randomly divided into normal, stress and Simotang group（ n= 10 in each group）, and given a variety of unpredictable chronic mild stress. After 21 days gastric emptying and intestinal propulsion function were measured,the expression of VIP was detected by immunohistochemistry and RT-PCR. Results Compared with mice in normal group（...