期刊论文

The protective effect of dexmedetomidine against focal cerebral ischemia/reperfusion injury in rats and its mechanism

中文

中华行为医学与脑科学杂志

Chinese Journal of Behavioral Medical Science

1673-923X

2016

25

2

117-121

10.3760/cma.j.issn.1674-6554.2016.02.005

本校为其他机构

目的研究右美托咪定(dexmedetomidine, Dex)对大鼠脑缺血/再灌注损伤的神经保护作用及其相关机制。方法雄性SD大鼠,采用右侧大脑中动脉栓塞90 min,再灌注24 h建立局灶性脑缺血/再灌注模型(I/R组),缺血即刻股静脉泵注Dex(15 μg ? kg~(-1)),根据需要在缺血前给予侧脑室注射PI3K阻断剂LY294002(LY,10 mM,10 μl)或内皮型一氧化氮合酶(eNOS)阻断剂L-NIO (1 mg ? kg~(-1),10 μl)。测定再灌注24 h后Dex对神经功能缺损评分,脑水肿,大脑梗死体积,缺血区神经元存活,缺血区脑组织p-Akt (Ser473)、p-eNOS(Ser1177)的蛋白表达。结果Dex处理组大鼠神经功能缺损评分、大脑梗死体积、脑水肿程度均显著低于单纯...

Objective To explore the neuroprotective effect of dexmedetomidine （Dex） in rats exposed to focal cerebral ischemia/reperfusion （I/R） induced by middle cerebral artery occlusion （MCAO） and the possible mechansims. Methods Adult male Sprague-Dawley rats were subjected to MCAO for 90 min followed by reperfusion for 24 h and Dex （ 15 ug·kg-1 ） was infused through the left femoral vein im- mediately after the onset of MCAO. The PI3K inhibitor LY294002 （LY, 10 mM, 10 ILl） or eNOS inhibitor L-NIO （ 1 mg· kg-1 , 10ul） was intracerebroven...