期刊论文

中文

中华行为医学与脑科学杂志

1674-6554

2017

26

8

684-688

10.3760/cma.j.issn.1674-6554.2017.08.003

基金项目：国家自然科学基金项目（81403379,81573965,81603604） 湖南省中药粉体与创新药物省部共建国家重点实验室培育基地开放基金项目（ZYFT201502）

本校为第一机构

目的探讨地佐环平(dizocilpine,MK801)对糖尿病并发抑郁症大鼠抑郁样行为及海马神经元损伤的影响。方法建立糖尿病并发抑郁症大鼠模型,并采用随机数字表将其随机分为2组:模型组、MK801组,以健康SD大鼠为正常对照组,每组6只。采用旷场实验检测大鼠行为学,采用 HE染色检查大鼠海马病理损伤;采用尼氏染色,检测大鼠海马神经元中的凋亡小体数目;采用 TUNEL染色,观察大鼠海马神经元凋亡;采用免疫荧光法,测定BaX、Bcl-2凋亡蛋白的表达。结果与正常组比较,模型组大鼠海马活动次数显著减少,海马神经元空泡、尼氏小体减少、凋亡细胞增多,凋亡蛋白 Bax 表达显著增加(94.57±7.97,30.08±5.73,P

Objective To investigate the effects of dizocilpine（ MK801 ） on depressive-like behav- iors and damaged hippocampus in rats with diabetes-related depression.Methods The animal models of dia- betes-related depression were established and they were randomly divided into two groups based on random number table： model group and MK801 group,while 6 rats were included in each group. And another six health rats were regarded as control group. The Open-field test was used to detect the activities. The damage of hippocampus was valued by HE staining,...