期刊论文

Regulation of thin recipe of Buyang Huanwudecoction on Cdk5 expression of rats after cerebral ischemia

中文

中国药理学通报

1001-1978

2017

33

8

1176-1181

10.3969/j.issn.1001-1978.2017.08.026

国家重点基础研究发展计划资助项目（No2012CB723503） 湖南省中医药科研计划项目（No2014135） 湖南省十二五中药学重点学科资助

本校为第一机构

目的评价补阳还五汤精简方对大脑中动脉阻塞(MCAO)模型大鼠海马组织周期素依赖性蛋白激酶5(cyclin-dependent kinase 5,Cdk5)的调控。方法将♂ SD大鼠随机分为假手术组、MCAO模型组、补阳还五汤干预组(灌胃3.15 g·kg~(-1))及补阳还五汤精简方干预组(灌胃2.41 g·kg~(-1)),每组按给药后1、3、7、14、28 d再各分5小组。采用免疫组化法、Western blot法和RT-PCR法考察不同时间点各组大鼠海马组织中Cdk5的表达。结果MCAO模型大鼠海马组织中,脑缺血后1 d即出现Cdk5上调趋势,并随着脑缺血的加剧,Cdk5的表达继续上调,其中mRNA及蛋白水平在7~14 d达最大,28 d则出现下降趋势,与各时间点假手术组比较P

Aim: To evaluate the regulation of thin recipe of Buyang Huanwu decoction on cyclin-dependent kinase 5 (Cdk5) expressions in hippocampus tissue of rats after cerebral ischemia. Methods: Male SD rats were divided into sham-operation group, MCAO group, Buyang Huanwu decoction group(ig. 3. 15 g • kg-1) and its thin recipe composition group (ig. 2.41 g • kg-1). Each group was then divided into five subgroups based on the time after administration for 1, 3, 7, 14, 28 d respectively. Cdk5 protein and mRNA levels in each group were examined by using...