期刊论文

Effect of tonifying kidney and invigorating spleen decoctions on dendritic cells in patients with hepatitis B virus infection in state of immune tolerance

中文

中西医结合肝病杂志

1005-0264

2010

20

4

221-224

10.3969/j.issn.1005-0264.2010.04.010

湖南省科技厅科技计划资助项目（No.2007FJ40） 湖南省自然科学基金资助项目（No.09JJ6042） 国家中医药管理局中医药科学技术研究专项资助项目（No.06-07JB17）

本校为第一机构

目的:比较补肾法和健脾法对慢性乙肝病毒(HBV)感染患者免疫耐受状态外周血树突状细胞(DCs)的影响.方法:收集20例慢性HBV感染免疫耐受期患者和10例健康人的抗凝外周静脉血,分离外周血单个核细胞(PBMCs),体外培养使DCs增殖、成熟,采用大鼠补肾和健脾药物血浆进行干预.检测DCs表面HLA-DR、CD-1α、CD80及CD86的表达,观察DCs培养上清液IL-12的水平及DCs刺激同种异体T细胞增殖能力.结果:健脾药物血浆及补肾药物血浆干预后CD80表达率升高,且补肾组的CD80表达率的提高优于健脾组(P＜0.05),而HLA-DR、CD-1α、CD86的表达水平在干预前后无显著性差异(P＞0.05).两组含药血浆干预后DCs刺激同种异体淋巴细胞...

Objective： To investigate the effect of TCM decoctions （ Liuweidihuang Tang and Sijunzi Tang） on dendritic cells （ DCs） in patients with chronic hepatitis B virus infection in state of immune tolerance. Methods： Twenty patients in im- mune tolerance stage with chronic hepatitis B virus infection and 10 healthy controls were included in this study. DCs subset popu- lations were differentiated from peripheral blood mononuclear cells （ PBMCs） ,incubated and induced then,the DCs were inter- fered by plasma of medicine of Liuweidihuang Tang ...