期刊论文

中文

中西医结合肝病杂志

1005-0264

2016

26

3

162-165

10.3969/j.issn.1005-0264.2016.03.014

本校为第一机构

目的:研究补肾解毒法对免疫耐受期产妇脐带血浆细胞样树突状细胞（pDCs）中Toll样受体9（TLR9）、干扰素调节因子7（IRF7）、α-干扰素（IFN-α）表达的影响。方法:选择HBV感染免疫耐受期产妇10例,体外培养脐带血pDCs。同时制备补肾方（六味地黄丸）、解毒方（甘露消毒丹）、补肾解毒方（六味地黄丸合甘露消毒丹）含药血浆。将培养第7d的pDCs分为空白组、补肾药物血浆组、解毒药物血浆组、补肾解毒药物血浆组、无药血浆组5组进行干预。培养48 h后Real-Time PCR检测pDCs中TLR9、IRF7 mRNA的表达;Western blot技术检测pDCs中TLR9、IRF7蛋白表达;ELISA法检测pDCs培养上清液中IFN-α水平。结果:补肾...

Objective： To study the effects of Bushen Jiedu decoction on expression of umbilical cord blood pDCs TLRg, IRF7, IFN-alpha on maternal infected with HBV during immune tolerance phase. Methods： Ten maternals infected with HBV during immune tolerance phase were enrolled in this study. Umbilical cord blood pDCs were cultured in vitro. At the same time, the plasma containing drug of Bushen recipe （liu wei di huang wan） , Jiedu recipe （gan lu xiao du dan） , Bushen Jiedu recipe （liu wei di huang wan and gan lu xiao du dan）was preparated. On t...