期刊论文

Protective effect of Panax notoginseng saponins on mitochondrial membrane potential of hippocampal neurons

中文

中华老年心脑血管病杂志

1009-0126

2008

10

12

941-943

10.3969/j.issn.1009-0126.2008.12.020

湖南省自然科学基金（07JJ6056） 湖南省教育厅科研项目（07C475）

本校为第一机构

目的 研究三七总皂苷对缺血再灌注后海马区神经元线粒体膜电位的保护作用.方法 36只SD大鼠随机分为假手术组、模型组、三七总皂苷组,每组12只.建立大鼠脑缺血再灌注模型,荧光染色流式细胞仪检测各组海马区神经元内游离Ca2+浓度和线粒体膜电位的变化.结果 与假手术组比较,模型组海马区神经元内游离Ca2+浓度明显升高,线粒体膜电位明显降低(P＜0.01);与模型组比较,三七总皂苷组海马区神经元内游离Ca2+浓度明显降低(P＜0.01),线粒体膜电位明显升高(P＜0.05).结论 三七总皂苷能抑制缺血再灌注引起的海马区神经元内Ca2+浓度升高,抑制线粒体膜电位下降,保护海马区神经元线粒体功能,这可能是其抗脑缺血再...

Objective To study the protective effect of Panax notoginseng saponins（PNS） on mitochondrial membrane potential（△ψm） of hippocampal neurons after cerebral ischemia-reperfusion. Methods Thirty-six rats were randomly divided into three groups,i, e. sham operation group,ischemia-reperfusion（model） group, PNS group. The model of cerebral ischemia-reperfusion was created by middle cerebral artery occlusion and recanalization. The concentration of free Ca^2＋ and △ψm of hippocampal neurons were assayed by flow cytometry. Results Compared wi...