期刊论文

Li, H.

中文

中国康复医学杂志

1001-1242

2009

24

10

880-883

10.3969/j.issn.1001-1242.2009.10.005

07JJ6056:湖南省自然科学基金 07C475:湖南省教育厅科学研究项目

本校为第一且通讯机构

目的:探讨活血通络组方对大鼠脑缺血再灌注损伤后神经功能恢复及其抗凋亡机制研究.方法:大脑中动脉闭塞再通法建立脑缺血再灌沣模型,采用神经行为学评分评价神经功能的恢复,跳台法测试大鼠学习和记忆成绩,原位术端标记法检测凋亡神经元,用免疫组化法检测Fas相关死亡结构域蛋白(FADD)、凋亡蛋白酶(caspase-8、caspase-3)阳性细胞数.结果:与模型组比较,活血通络组的学习和记忆成绩均优于模型组,神经功能损伤评分亦有明显降低,神经元凋亡指数(AI)、FADD、caspase-8、caspase-3阳性细胞数均显著减少.结论:FADD、caspase-8、caspase-3在脑缺血再灌注后细胞凋亡过程中发挥重要作用,活血通络组方能减轻...

Objective: To study the effects of huoxuetongluo recipe on neural function recovery after cerebral ischemia reperfusion injury in rats and its mechanism of anti-apoptosis. Method: The model of cerebral ischemia reperfusion (I/R) was induced by middle cerebral artery occlusion (MCAO) and recanalization. Terminal deoxyribo-nucleotide transferase-mediated dUTP-biotin nick end labeling TUNEL) method was used to detect the apoptotic neurocytes. The positive neurons of Fas associated with death domain protein(FADD),caspase-8 and caspase-3 were detected by immunohistochemistry. Result: Compared with ...