期刊论文

中文

湖南中医药大学学报

1674-070X

2004

24

2

4-6

10.3969/j.issn.1674-070X.2004.02.002

湖南省教委资助项目 （A0 0 64 ）；

本校为第一机构

目的探讨补阳还五汤对缺氧 - 复氧内皮细胞ICAM - 1mRNA表达的影响及其保护作用.方法取内皮细胞株EVC304,随机分为5组进行相应处理.空白组(A)37 ℃, 5%CO2正常培养24 h;缺氧组(B)持续通入95%N2+5%CO2混合气24 h;缺氧 - 复氧组(C)持续通入95%N2+5%CO2混合气12 h后,改用正常培养12 h;西药组(D)实验前加入终浓度为10 ng /L的尼莫地平,方法同C组;补阳还五汤组(E):以补阳还五汤培养,方法同C组.硫氰酸胍 - 酚 - 氯仿法提取各组细胞总RNA,以β - Actin为内对照,将RT - PCR产物用1.5%的琼脂糖凝胶电泳,用ICAM - 1和β - Actin的光密度比值表示mRNA水平.结果缺氧 - 复氧组ICAM - 1mRNA表达最高(P

Objective To investigate the effects of Buyang Huwan Decoction(BHD) on the expression of intercellular adhesion molecule-1 (ICAM-1) mRNA in the endothelial cell line EVC304 cultured in hypoxic-reoxgenative circumstance. Methods The cuturled cells of ECV304 were randomly divided into 5 groups, i.e. blank group (A), hypoxic group (B), hypoxic-reoxgenative group (C),drug controlling group (D) and BHD group (E), all being treated by corresponding conditions respectively. Then, the expression of ICAM-1 mRNA was determined by reverse transcriptase polymerase chain reaction (RT-PCR). Results The high...