期刊论文

中文

中药药理与临床

1001-859X

2013

29

5

52-56

10.13412/j.cnki.zyyl.2013.05.019

国家“十二五”“重大新药创制”科技重大专项项目

本校为第一机构

目的:考察白头翁皂苷对人结肠癌细胞株HT29的增殖及诱导细胞凋亡的作用.方法:MTT法检测白头翁皂苷对HT29细胞增殖的影响;DAPI染色、Annexin-V/PI染色流式细胞术检测对细胞凋亡的影响;Western blotting检测白头翁皂苷对凋亡相关蛋白cleaved-caspase3的影响.结果:白头翁皂苷能明显抑制HT29细胞的增殖,其20、10、5、2.5、1.25、0.625 μg/ml浓度作用48h对HT29增殖抑制率依次为97.6％、87.1％、76.0％、67.3％、59.4％、41.9％,呈浓度依赖关系,IC50值为1.440μg/ml;DAPI染色可以观察到白头翁皂苷干预后的HT29细胞出现明显的凋亡形态;流式细胞术检测白头翁皂苷5、10、15、20μg/ml诱导HT29细胞凋亡率...

This study was to investigate the effect of Pulsatilla saponins on proliferation and apoptosis of HT29 cells. Methods： HT29 cells were treated with Pulsatilla saponins,cell viability was assessed by MTT assay; apoptosis were observed by DAPI staining,the apoptosis ratio were assessed by flow cytometry after Annexin V/PI staining;Cleaved-caspase3 were detected by Western Blotting assay. Result： Pul- satilla saponins could inhibit the proliferation of HT29 cells in a manner of dosedepedence, the inhibition rate of proliferation at the dose of 2...