期刊论文

中文

中药药理与临床

1001-859X

2015

31

4

183-187

10.13412/j.cnki.zyyl.2015.04.057

12k090:湖南省教育厅高等学校创新平台开放基金

本校为第一机构

目的:研究丹龙醒脑方对脑缺血再灌注损伤大鼠大脑海马区血管内皮生长因子(Vascular endothelial growth factor, VEGF)及其受体血管内皮生长因子受体(Fetal liver kinase -1,Flk-1)的影响。方法:用改良Longa栓线法复制大脑中动脉闭塞模型,2h后开放血流再灌注。大鼠造模成功后被分为6组;丹龙醒脑方14.76g /kg、7.38g /kg、3.69g /kg剂量组,尼莫地平10.8mg /kg组,模型组,假手术组,灌胃7天后处死大鼠取脑,采用免疫组化方法测定海马区内皮细胞计数及微血管密度、VEGF、Flk-1的表达。结果:模型组与假手术组相比,大鼠脑海马区内皮细胞计数及微血管密度、VEGF、Flk-1蛋白表达均增强;丹龙醒脑方14.76g /...

Objective： To investigate Danlong-Xingnao formula effect on VEGF and Flk-1 of cerebral ischemia-reperfusion injury rats hippocampus.Methods： The MCAO rat model was created by modified Longa method,blood reperfusion opened after 2 hours. The rats were randomly divided into six groups（ High-dose,moderate-dose and low-dose Danlong-Xingnao groups,nimodipine group,model group and Sham group）.The rats were given by gavage once a day for 7 days. Microvessel density,VEGF and Flk-1 of rats hippocampus were detected by immunohistochemistry. Results：...