期刊论文

Effect of Zuogui Jiangtang Jieyu Formula on hippocampal neuron NR-5-HT in Diabetes Mellitus Complicated with Depression Cell Model

中文

中药药理与临床

1001-859X

2018

34

3

137-141

10.13412/j.cnki.zyyl.2018.03.033

湖南省研究生科研创新项目（2017CX12） 一方研究生科研创新项目（YF201702） 湖南省自然科学基金项目（2017JJ3235） 湖南省教育厅优秀青年资助项目（17B201）

本校为第一机构

目的:研究左归降糖解郁方(ZGJTJYF)对模拟糖尿病并发抑郁症(Diabetes mellitus with depression,DD)环境下大鼠胎鼠海马神经元N-甲基-D-天冬氨酸受体(N-methyl-D-aspartic acid receptor,NR) -5-羟色胺(5-hydroxytryptamine,5-HT)信号轴的影响。方法:分离、纯化、培养并鉴定胎鼠海马神经元,将培养的海马神经元随机分为6组:空白组、空白血清组、模型组、氟西汀+二甲双胍阳性药组、左归降糖解郁方组和NR阻断剂MK-801组。除空白组和空白血清组外,其余各组分别给予葡萄糖(150 mmol /L)和皮质酮(200 μmol /L)用以构建DD海马神经元体外模型,氟西汀+二甲双胍阳性药组和左归降糖解郁方组分别给予10%相应...

Objective: To research the effect of Zuogui Jiangtang Jieyu Formula (ZGJTJYF) on N-methyl-D-aspartate acid receptor (NR) -5- hydroxytryptamine (5-HT) signal axis in fetal rat's hippocampus neuron with diabetes mellitus complicated with depression (DD) . Methods: Neurons (NE) were separated,purified,cultured and identified. Cultured hippocampal neurons were randomly divided into 6 groups: the control group,the serum group,the model group,melbine & fluoxetine serum group,ZGJTJF group and blocking agent MK-801 group. Except the control and serum g...