期刊论文

Effect of PNS pretreatment on the expressions of AP-1 and TGF-β1 in renal tissues of rats with renal ischemia-reperfusion injury

中文

中药药理与临床

1001-859X

2018

34

6

73-78

10.13412/j.cnki.zyyl.2018.06.019

国家自然科学基金项目(No.81503442) 湖南省教育厅科研项目(No.17B197) 湖南中医药大学大学生研究性学习和创新性实验课题计划(2018-48).

本校为第一机构

目的:探讨三七总皂苷(PNS)预处理对大鼠肾缺血再灌注损伤及肾组织AP-1、TGF-β1表达的影响。方法: SD雄性大鼠30只,随机分成假手术组、肾缺血再灌注损伤组、三七总皂苷低、中、高剂量组和缬沙坦组。给予假手术组及肾缺血再灌注损伤组生理盐水(10mL/kg)灌胃,其余四组分别给予40、80、160mg/kg三七总皂苷和55mg/kg缬沙坦灌胃,1次/天,连续给药5天,末次给药1h后造模,切除右肾,夹闭左肾动脉缺血45min再灌注4h完成造模,假手术组仅分离左肾动、静脉,不夹闭。术后处死大鼠,检测大鼠血肌酐(SCr)、血尿素氮(BUN)、肾组织丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)含量,HE染色切片观测...

Objective: To explore the effects of pretreatment with Panax notoginseng saponins (PNS) on the expressions of AP-1 and TGF-β1 in renal tissues of rats with renal ischemia-reperfusion injury. Methods: 30 SD male rats were randomly divided into the sham operation group, renal ischemia reperfusion injury group,three doses of panax notoginseng saponins groups and Valsartan group,5 rats in each group. Rats in the sham operation group and renal ischemia reperfusion injury group were administered with normal saline (10 mL/kg) ,rats in the remaining f...