期刊论文

Effect of Juanbilijieqingfang on main components of the TLR4 /NF-κB signaling pathway in gout cell model

中文

中药药理与临床

1001-859X

2019

35

1

155-160

10.13412/j.cnki.zyyl.2019.01.034

国家自然科学基金项目(81574005) 湖南省中医药研究院院级科研课题重点课题(201807) 湖南省研究生科研创新课题(CX2015B317)。

本校为第一机构

目的:研究蠲痹历节清方对痛风作用及对TLR4 /NF-κB信号通路主要元件的影响。方法:实验分为两部分,①不添加TLR4受体抑制剂部分;②添加TLR4受体抑制剂部分。每部分利用单钠尿酸盐( MSU)结晶诱导巨噬细胞产生痛风模型,随机分正常对照组、模型组、蠲痹历节清方组,并分别干预。用Westen-blot法测定干预后巨噬细胞中髓样分化分子88( MyD88) 、IκB激酶-β( IKK-β) 、NF-κB抑制蛋白α( IκB-α)蛋白表达,RT-PCR测定干预后各组巨噬细胞中Toll样受体4( TLR4)基因表达;免疫荧光法检测巨噬细胞核转录因子kappa B( NF-κB)核移位情况; ELISA法检测白细胞介素-1β( IL-1β) 、白细胞介素-6( IL-6) 、肿瘤...

Objective: To study the effect of Juanbilijieqingfang on gout cell and its main components in the TLR4 /NF-κB signaling pathway. Methods: The experiment was divided into two parts: 1. No addition of TLR4 receptor inhibitor. 2. Addition of TLR4 receptor inhibitor. In each part,gout model was established by MSU to simulate the macrophage,then cells were randomly divided into 3 groups, the normal control group, the model group and Juanbilijieqingfang group. In this study,the protein contents of MyD88,IKK-β,IκB-α in macrophages of each group we...