期刊论文

The Role of Sema4D in Airway Remodeling Induced by Virus in Asthmatic Rats and the Intervention of Kechuanning(咳喘宁)

中文

中药药理与临床

1001-859X

2019

35

6

116-120

10.13412/j.cnki.zyyl.2019.06.025

NO.81674025:国家自然科学基金 81774368:国家自然科学基金 NO.2015JJ2016:湖南省自然科学基金

本校为第一机构

目的:探讨脑信号蛋白4D(Semaphorins4D,Sema4D)对呼吸道合胞病毒(Respiratory syncytial virus,RSV)诱发哮喘大鼠气道重塑的影响及咳喘宁的改善作用。方法:构建RSV复合卵清白蛋白(Ovalbumin,OVA)模型,实验大鼠随机分为模型组、咳喘宁0.2 g/kg、2.1 g/kg、7 g/kg组、Sema4D抗体0.01 mg/kg组,另设正常对照组,灌胃予以相应药物或生理盐水干预,连续2 w。检测大鼠气道反应性,HE染色观察各组大鼠肺组织病理改变;Masson染色观察各组大鼠肺组织胶原沉积状况;免疫组织化学观察各组大鼠肺组织α-平滑肌肌动蛋白(α-smooth muscle actin,α-SMA) 、基质金属蛋白酶-2(matrix metalloprotein-2,MMP-2)的表达;...

Objective:To investigate the effect of Sema4 D on airway remodeling induced by RSV in asthmatic rats and the improvement of Kechuanning.Methods:RSV combined with OVA model was established.The rats were randomly divided into the control group,the model group,0.2 g/kg,2.1 g/kg,7 g/kg Kechuanning groups,and 0.01 mg/kg Sema4 D antibody group,and given corresponding drug intervention or saline water for 2 weeks constantly.The airway responsiveness of rats was measured,HE staining was used to observe pathological changes of lung tissues in rats,the c...