期刊论文

Model establishment and evaluation of rat duodenal ulcer with Piweixuhan syndrome based on the theory of testing syndrome by prescription

中文

中药药理与临床

1001-859X

2019

35

1

183-188

10.13412/j.cnki.zyyl.2019.01.041

国家自然科学基金项目(81703920) 湖南省中医药管理局一般项目(201780) 湖南省大学生研究性学习和创新性实验计划项目(2017280).

本校为第一机构

目的:基于以方测证理论构建可靠、稳定的十二指肠溃疡( DU)脾胃虚寒证候大鼠实验模型,并对模型进行综合评价。方法:将48只SD大鼠随机分为正常组、模型组、柴胡疏肝散6.56g /kg组(非方证对应组)和黄芪建中汤9.27g /kg组(方证对应组) 。采用苦寒泻下加劳倦过度法构建脾胃虚寒证候,运用阿司匹林加无水乙醇建立DU模型。观测大鼠的整体状态、体质量、进食量和肛温变化,计算溃疡指数( UI),HE染色观察十二指肠黏膜病理改变,ELISA法检测血清PGE、NO、MDA含量,免疫组化法测定TLR-2、MyD88蛋白表达。结果:与正常组比较,模型组大鼠体质量、进食量、肛温均明显降低。给予黄芪建中汤治疗后与模型组比较,大鼠整...

Objective: To establish a reliable and stable experimental model of duodenal ulcer rat with Piweixuhan syndrome and evaluate the model. Methods: 48 SD rats were randomly divided into the normal group, the model group,6. 56g /kg Chaihu Shugan San group,and 9.27g /kg Huangqi Jianzhong Decoction( HQJZ) group. Piweixuhan syndrome model was established by using methods of " bitter cold induced diarrhea" and " overstrain". Duodenal ulcer model was established by using absolute ethyl alcohol and aspirin. The general symptoms and signs of rats were observed and the changes of body weight,food intake a...