期刊论文

Effect of Jiangzhi Ligan Decoction on SREBP-1c Pathway in the Nonalcoholic Fatty Liver Disease (NAFLD) Cell Model Induced in Vitro

中文

中药药理与临床

1001-859X

2019

35

6

149-153,175

10.13412/j.cnki.zyyl.2019.06.032

国家自然科学基金项目(81503385) 2017年湖南省卫计委科研计划项目(C2017028) 2018年湖南省教育厅科学研究项目(18B244,18B252) 湖南中医药大学"十三五"一级学科基础医学建设项目(06).

本校为第一机构

目的:观察降脂理肝汤对油酸诱导的HepG2细胞中固醇调节元件结合蛋白1c(sterol regulatory element binding protein-1c,SREBP-1c)通路相关蛋白表达的影响,探讨降脂理肝汤治疗非酒精性脂肪肝的可能机制。方法:采用油酸诱导HepG2细胞建立非酒精性脂肪肝细胞模型,降脂理肝汤4 mg/ml和阿托伐他汀0.005 mg/ml干预后,利用油红O染色定性定量检测细胞内脂质蓄积情况;试剂盒检测细胞内TG、TC和FFA水平;Western bolt观察和分析细胞p-SREBP-1c、SREBP-1c、FAS、ACC1和SCD1蛋白表达的变化。结果:油酸诱导的HepG2细胞脂质蓄积明显;细胞内TG、TC和FFA含量明显升高,细胞p-SREBP-1c蛋白表达水平显著下调,SREBP-1...

Objective: To study the effect of Jiangzhi Ligan Decoction on SREBP-1c pathway in cell model with nonalcoholic fatty liver disease(NAFLD), thus to explore the possible mechanism of Jiangzhi Ligan Decoction on NAFLD.Methods: HepG2 cells model with NAFLD was established by oleic acid (OA).After the intervention of Jiangzhi Ligan Decoction(4 mg/ml) or Atorvastatin(0.005 mg/ml), the lipid accumulation was detected qualitatively and quantitatively by oil red O staining,the levels of TG,TC and FFA were measured by the detection kits,the protein expressions levels of p-SREBP-1c,SREBP-1c,FAS,ACC1 and ...