期刊论文

Experimental study of Jinshuiliujunjian on the treatment of COPD and its antagonism against dexamethasone

中文

中药药理与临床

1001-859X

2019

35

3

19-24

10.13412/j.cnki.zyyl.2019.03.004

国家自然科学基金项目（81473564）；

本校为第一机构

目的:探讨金水六君煎治疗慢性阻塞性肺疾病(COPD)阴虚痰饮的作用和机制。方法: SD大鼠60只,随机分成空白组、模型组、地塞米松0. 5 mg /kg组、地塞米松+金水六君煎2. 62、5. 25、10. 5 g /kg组。采用烟熏联合气道脂多糖滴入建立COPD大鼠模型,地塞米松肌注诱导大鼠阴虚状态。观察大鼠一般状态、肺功能、酚红排泌量,PAS染色法测定气道杯状细胞数,RT-PCR、 Western法检测肺组织黏蛋白5AC(MUC5AC)和水通道蛋白5(AQP5)基因与蛋白表达,ELISA法测定血清ACTH、CORT、cAMP、 cGMP、cAMP/cGMP含量。结果:模型组大鼠肺功能明显下降,气道杯状细胞数、酚红排泌量及MUC5AC表达明显增多,AQP5表达明显下降。地塞...

Objective: To explore the effect of Jinshuiliujunjian on COPD and its Yinxutanyin mechanism. Methods: 60 SD rats were randomly divided into the control group,the model group, 0.5 mg/kg dexamethasone group, dexamethasone+ Jinshuiliujunjian combination groups(2.62,5.25,10.5 g/kg). COPD model was established by cigarette smoking combined with LPS instillation in airway. Yinxu symptoms of rats was established by intramuscular injection of dexamethasone. Rats general state, lung function and phenol red excretion were observed. PAS staining was used to determine the number of goblet cells and mucus ...