期刊论文

Effect of RSV-Induced Dendritic Cells Autophagy on Airway Responsiveness in Asthmatic Mice and Intervention of Wuhu Decoction

中文

中药药理与临床

1001-859X

2020

36

04

59-65

10.13412/j.cnki.zyyl.2020.04.001

国家自然科学基金项目（编号:81774368）； 湖南省自然科学基金科卫基金（编号:2018JJ6114）；

本校为第一机构

目的:探讨呼吸道合胞病毒诱导的树突细胞自噬对哮喘小鼠气道反应性的影响及五虎汤的干预作用。方法: 160只SPF级雌性BALB/c小鼠,用呼吸道合胞病毒(Respiratory syncytial virus,RSV)复合鸡卵蛋白(Ovalbumin,OVA)雾化吸入激发哮喘,造模成功后随机分为正常对照组、模型对照组、地塞米松1.8 mg/kg组、雷帕霉素1 mg/kg组及五虎汤6.4 g/kg、3.2 g/kg、1.6 g/kg组,并予以相应药物干预两周。使用动物呼吸机检测小鼠气道反应性值; HE染色法观察小鼠肺组织病理学改变; Masson和PAS染色法观察气道胶原沉积和粘液储备情况; Western Blot法测定α-平滑肌肌动蛋白(α-smooth muscle actin,α-SMA) 、转化生长...

Objective: To investigate the effect of respiratory syncytial virus-induced dendritic cell autophagy on the airway responsiveness of asthmatic mice and the intervention effect of Wuhu Tang. Methods: 160 SPF female BALB/c mice were inhaled with respiratory syncytial virus (RSV) combined with chicken egg protein (Ovalbumin,OVA) to establish the asthma model. After successful modeling,mice were randomly divided into the control group, the model group,1.8 mg/kg dexamethasone group,1 mg/kg rapamycin group and Wuhu decoction groups (6.4 g/kg,3.2 g/kg...